Format

Send to

Choose Destination
Mol Biol Evol. 2016 Apr;33(4):898-914. doi: 10.1093/molbev/msv280. Epub 2015 Nov 26.

Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans.

Author information

1
Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France French National Reference Center for Bacterial Resistance in Commensal Flora, Laboratory of Bacteriology, Bichat-Claude Bernard Hospital, Assistance Publique-Hôpitaux De Paris, Paris, France dskurnik@rics.bwh.harvard.edu erick.denamur@inserm.fr.
2
INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France.
3
Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.
4
Harvard Medical School, Boston, MA.
5
Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France.
6
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
7
Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France.
8
Harvard School of Public Health, Boston, MA.
9
INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France French National Reference Center for Bacterial Resistance in Commensal Flora, Laboratory of Bacteriology, Bichat-Claude Bernard Hospital, Assistance Publique-Hôpitaux De Paris, Paris, France.
10
INSERM, IAME, UMR 1137, Paris, France Univ Paris Nord, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France.
11
INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France dskurnik@rics.bwh.harvard.edu erick.denamur@inserm.fr.

Abstract

In the context of the great concern about the impact of human activities on the environment, we studied 403 commensal Escherichia coli/Escherichia clade strains isolated from several animal and human populations that have variable contacts to one another. Multilocus sequence typing (MLST) showed a decrease of diversity 1) in strains isolated from animals that had an increasing contact with humans and 2) in all strains that had increased antimicrobial resistance. A specific B1 phylogroup clonal complex (CC87, Institut Pasteur schema nomenclature) of animal origin was identified and characterized as being responsible for the increased antimicrobial resistance prevalence observed in strains from the environments with a high human-mediated antimicrobial pressure. CC87 strains have a high capacity of acquiring and disseminating resistance genes with specific metabolic and genetic determinants as demonstrated by high-throughput sequencing and phenotyping. They are good mouse gut colonizers but are not virulent. Our data confirm the predominant role of human activities in the emergence of antimicrobial resistance in the environmental bacterial strains and unveil a particular E. coli clonal complex of animal origin capable of spreading antimicrobial resistance to other members of microbial communities.

KEYWORDS:

Escherichia coli; antimicrobial resistance; clonal complex 87; commensal; phylogroup B1

PMID:
26613786
PMCID:
PMC5013867
DOI:
10.1093/molbev/msv280
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center