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Sci Rep. 2015 Nov 27;5:17322. doi: 10.1038/srep17322.

Formaldehyde scavengers function as novel antigen retrieval agents.

Author information

1
Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77204, USA.
2
Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.

Abstract

Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging improves the typical antigen retrieval process through reversal of formaldehyde-protein adduct formation. This approach provides a rational framework for the identification and development of more effective antigen retrieval agents.

PMID:
26612041
PMCID:
PMC4661444
DOI:
10.1038/srep17322
[Indexed for MEDLINE]
Free PMC Article

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