Format

Send to

Choose Destination
Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j. Epub 2015 Nov 27.

A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection.

Author information

1
Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA 95616, USA. pcronald@ucdavis.edu.

Abstract

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

PMID:
26611838
PMCID:
PMC5061041
DOI:
10.1039/c5ib00232j
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center