Format

Send to

Choose Destination
Toxicon. 2016 Feb;110:47-50. doi: 10.1016/j.toxicon.2015.11.007. Epub 2015 Dec 2.

In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions.

Author information

1
ANSES, French Agency for Food, Environmental and Occupational Health Safety, Fougères Laboratory, 10B rue Claude Bourgelat - Javené, CS 40608, 35306, Fougères Cedex, France; Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
2
ANSES, French Agency for Food, Environmental and Occupational Health Safety, Fougères Laboratory, 10B rue Claude Bourgelat - Javené, CS 40608, 35306, Fougères Cedex, France.
3
Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany; Leibniz-Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V., Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany.
4
Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, 10589, Berlin, Germany.
5
ANSES, French Agency for Food, Environmental and Occupational Health Safety, Fougères Laboratory, 10B rue Claude Bourgelat - Javené, CS 40608, 35306, Fougères Cedex, France. Electronic address: valerie.fessard@anses.fr.

Abstract

No evidence for phase I metabolites of the cyanotoxin cylindrospermopsin (CYN) was given using HepaRG cells and different liver tissue fractions when studying metabolic conversion. Although the application of ketoconazole, a CYP3A4 inhibitor, led to a decreased cytotoxicity of CYN, no metabolites were detected applying high resolution mass spectrometry. Quantification of non-modified CYN led to recovery rates of almost 100%. Consequently, reduction of CYN toxicity in the presence of metabolism inhibiting agents must be attributed to alternative pathways.

KEYWORDS:

Cylindrospermopsin; HepaRG cells; LC-HRMS; Liver tissue fractions; Metabolism; Quantification

PMID:
26610396
DOI:
10.1016/j.toxicon.2015.11.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center