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Methods Mol Biol. 2016;1379:99-105. doi: 10.1007/978-1-4939-3191-0_9.

Comet Assay in Cancer Chemoprevention.

Author information

1
Molecular Chemoprevention Unit, Molecular Medicine Area, Regina Elena National Cancer Institute, via Elio Chianesi 53, 00144, Rome, Italy. r.santoro@activep53.eu.
2
Molecular Chemoprevention Unit, Molecular Medicine Area, Regina Elena National Cancer Institute, via Elio Chianesi 53, 00144, Rome, Italy.
3
Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, ON, Canada.
4
Department of Oncology, Juravinski Cancer Center, McMaster University, Hamilton, ON, Canada, L8V 5C2.
5
Molecular Cancer Chemoprevention Unit, Molecular Medicine Department, Regina Elena National Cancer Institute, via Elio Chianesi 53, 00144, Rome, Italy.
6
Department of Oncology, Juravinski Cancer Center-McMaster University Hamilton, Hamilton, ON, Canada, L8V 5C2.

Abstract

The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

KEYWORDS:

Comet assay; DNA damage; DNA repair; Double-strand breaks (DSB); Genotoxic stress; Single-cell gel electrophoresis (SCGE); Single-strand breaks (SSB)

PMID:
26608293
DOI:
10.1007/978-1-4939-3191-0_9
[Indexed for MEDLINE]

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