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J Microbiol Biotechnol. 2016 Mar;26(3):579-87. doi: 10.4014/jmb.1510.10070.

Phytoncide Extracted from Pinecone Decreases LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells.

Author information

1
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
2
Department of Animal Science and Technology, Konkuk University, Seoul 05029, Republic of Korea.
3
Wide River Institute of Immunology, Seoul National University, Hongcheon 25159, Republic of Korea.
4
Toxicology and Cancer Biology, College of Medicine, University of Kentucky, Lexington, KY 40536, USA.
5
Phylus Corporation, Danyang 27000, Republic of Korea.
6
Department of Animal Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
7
Department of Biotechnology, CHA University, Seongnam 13488, Republic of Korea.

Abstract

Mastitis is a prevalent inflammatory disease that remains one of the main causes of poor quality of milk. Phytoncides are naturally occurring anti-inflammatory compounds derived from plants and trees. To determine if treatment with phytoncide could decrease the severity of lipopolysaccharide (LPS)-induced inflammatory responses, mammary alveolar epithelial cells (MAC-T) were pretreated with phytoncide (0.02% and 0.04% (v/v)) followed by LPS treatment (1 and 25 μg/ml). The results demonstrated that phytoncide downregulated LPS-induced pro-inflammatory cyclooxygenase-2 (COX-2) expression. Additionally, LPS-induced activation of ERK1/2, p38, and Akt was attenuated by phytoncide. Treatment of cells with known pharmacological inhibitors of ERK1/2 (PD98059), p38 (SB203580), and Akt (LY294002) confirmed the association of these signaling pathways with the observed alterations in COX-2 expression. Moreover, phytoncide attenuated LPS-induced NF-κB activation and superoxide production, and, finally, treatment with phytoncide increased Nrf2 activation. Results suggest that phytoncide can decrease LPS-induced inflammation in MAC-T cells.

KEYWORDS:

COX-2; MAC-T cell; inflammation; lipopolysaccharide; mastitis

PMID:
26608166
DOI:
10.4014/jmb.1510.10070
[Indexed for MEDLINE]
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