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Methods. 2016 Mar 1;96:97-102. doi: 10.1016/j.ymeth.2015.11.013. Epub 2015 Nov 21.

High-throughput imaging: Focusing in on drug discovery in 3D.

Author information

1
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, United States.
2
PerkinElmer Inc., 940 Winter Street, Waltham, MA 02451, United States.
3
Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, United States; The University of Colorado Cancer Center, Developmental Therapeutics Program, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, United States. Electronic address: Daniel.LaBarbera@ucdenver.edu.

Abstract

3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery and toxicology screening. As a result, 3D culture technologies adapted for high-throughput screening formats are prevalent. While a multitude of assays have been reported and validated for high-throughput imaging (HTI) and high-content screening (HCS) for novel drug discovery and toxicology, limited HTI/HCS with large compound libraries have been reported. Nonetheless, 3D HTI instrumentation technology is advancing and this technology is now on the verge of allowing for 3D HCS of thousands of samples. This review focuses on the state-of-the-art high-throughput imaging systems, including hardware and software, and recent literature examples of 3D organotypic culture models employing this technology for drug discovery and toxicology screening.

KEYWORDS:

3D image analysis software; 3D tissue culture; High-content screening; High-throughput imaging; Multicellular tumor spheroids; Organoids

PMID:
26608110
PMCID:
PMC4766031
[Available on 2017-03-01]
DOI:
10.1016/j.ymeth.2015.11.013
[Indexed for MEDLINE]
Free PMC Article

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