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PLoS One. 2015 Nov 25;10(11):e0142989. doi: 10.1371/journal.pone.0142989. eCollection 2015.

Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.

Author information

1
Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.
2
Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
3
Agence de Médecine Préventive (AMP), Paris, France.
4
Institut National d'Hygiène, Lomé, Togo.
5
Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of Congo.
6
Institut National de Santé Publique, Conakry, Guinea.
7
Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire.
8
National Institute of Health, Maputo, Mozambique.

Abstract

We used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for cholera toxin. All isolates showed multidrug resistance but lacked ciprofloxacin resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to ceftriaxone and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of cholera epidemiology in Africa.

PMID:
26606536
PMCID:
PMC4659613
DOI:
10.1371/journal.pone.0142989
[Indexed for MEDLINE]
Free PMC Article

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