Format

Send to

Choose Destination
Biomaterials. 2016 Jan;77:255-66. doi: 10.1016/j.biomaterials.2015.10.026. Epub 2015 Oct 22.

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds.

Author information

1
Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA.
2
Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA; Research Service, Edward Hines Jr. V.A. Hospital, Hines, IL, USA.
3
Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
4
Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA. Electronic address: David.Dean@osumc.edu.

Abstract

The importance of vascularization in the field of bone tissue engineering has been established by previous studies. The present work proposes a novel poly(propylene fumarate) (PPF)/fibrin composite scaffold for the development of vascularized neobone tissue. The effect of prevascularization (i.e., in vitro pre-culture prior to implantation) with human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on in vivo vascularization of scaffolds was determined. Five conditions were studied: no pre-culture (NP), 1 week pre-culture (1P), 2 week pre-culture (2P), 3 week pre-culture (3P), and scaffolds without cells (control, C). Scaffolds were implanted subcutaneously in a severe combined immunodeficiency (SCID) mouse model for 9 days. During in vitro studies, CD31 staining showed a significant increase in vascular network area over 3 weeks of culture. Vascular density was significantly higher in vivo when comparing the NP and 3P groups. Immunohistochemical staining of human CD-31 expression indicated spreading of vascular networks with increasing pre-culture time. These vascular networks were perfused with mouse blood indicated by perfused lectin staining in human CD-31 positive vessels. Our results demonstrate that in vitro prevascularization supports in vivo vascularization in PPF/fibrin scaffolds.

KEYWORDS:

Fibrinogen; Human umbilical vein endothelial cells (HUVECs); Mesenchymal stem cells (MSCs); Poly(propylene fumarate) (PPF); Thrombin

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center