Protective Effects of Lactobacillus plantarum CCFM8246 against Copper Toxicity in Mice

Fengwei Tian; Yue Xiao; Xiaoxiao Li; Qixiao Zhai; Gang Wang; Qiuxiang Zhang; Hao Zhang; Wei Chen

doi:10.1371/journal.pone.0143318

Protective Effects of Lactobacillus plantarum CCFM8246 against Copper Toxicity in Mice

PLoS One. 2015 Nov 25;10(11):e0143318. doi: 10.1371/journal.pone.0143318. eCollection 2015.

Authors

Fengwei Tian¹, Yue Xiao¹, Xiaoxiao Li¹, Qixiao Zhai¹, Gang Wang¹, Qiuxiang Zhang¹, Hao Zhang¹, Wei Chen^{1

2}

Affiliations

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People's Republic of China.
² Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, People's Republic of China.

Abstract

Lactobacillus plantarum CCFM8246, which has a relatively strong copper binding capacity and tolerance to copper ions, was obtained by screening from 16 lactic acid bacteria in vitro. The selected strain was then applied to a mouse model to evaluate its protective function against copper intoxication in vivo. The experimental mice were divided into an intervention group and a therapy group; mice in the intervention group received co-administration of CCFM8246 and a copper ion solution by gavage, while mice in the therapy group were treated with CCFM8246 after 4 weeks of copper exposure. In both two groups, mice treated with copper alone and that treated with neither CCFM8246 nor copper served as positive and negative controls, respectively. At the end of the experimental period, the copper content in feces and tissues, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, and oxidation stress indices in liver and kidney tissue were determined. Learning and memory ability was evaluated by Morris water maze experiments. The results indicated that treatment with CCFM8246 significantly increased the copper content in feces to promote copper excretion, reduce the accumulation of copper in tissues, reverse oxidative stress induced by copper exposure, recover the ALT and AST in serum and improve the spatial memory of mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal
Bile Acids and Salts / metabolism
Bile Acids and Salts / pharmacology
Brain / drug effects
Brain / metabolism
Brain / pathology
Copper / metabolism*
Copper / toxicity*
Disease Models, Animal
Gastric Juice / metabolism
Ions / metabolism
Ions / toxicity
Kidney / drug effects
Kidney / metabolism
Kidney / pathology
Lactobacillus plantarum / drug effects
Lactobacillus plantarum / metabolism*
Liver / drug effects
Liver / metabolism
Liver / pathology
Liver Function Tests
Male
Maze Learning
Mice
Microbial Viability / drug effects
Oxidative Stress
Toxicity Tests

Substances

Bile Acids and Salts
Ions
Copper

Grants and funding

This work was supported by the National Natural Science Foundation of China Key Program (No. 31530056, WC), the National Science Fund for Distinguished Young Scholars (No. 31125021, WC), the National Natural Science Foundation of China General Program (No. 31470161, WC), the Science and Nature Foundation of Jiangsu Province (No. BK 20131102, WC), the key projects in the national science & technology pillar program during the twelfth five-year plan period (No. 2012BAD12B02, 2012BAD28B08, WC), the National Basic Research Program of China (973 Program No. 2012CB720802, WC), the 111 Project B07019, and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1249, WC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.