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Cancer Lett. 2016 Feb 1;371(1):48-61. doi: 10.1016/j.canlet.2015.10.020. Epub 2015 Nov 18.

Bovine milk-derived exosomes for drug delivery.

Author information

1
Department of Medicine, University of Louisville, Louisville, KY 40202.
2
James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202.
3
Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202.
#
Contributed equally

Abstract

Exosomes are biological nanovesicles that are involved in cell-cell communication via the functionally-active cargo (such as miRNA, mRNA, DNA and proteins). Because of their nanosize, exosomes are explored as nanodevices for the development of new therapeutic applications. However, bulk, safe and cost-effective production of exosomes is not available. Here, we show that bovine milk can serve as a scalable source of exosomes that can act as a carrier for chemotherapeutic/chemopreventive agents. Drug-loaded exosomes showed significantly higher efficacy compared to free drug in cell culture studies and against lung tumor xenografts in vivo. Moreover, tumor targeting ligands such as folate increased cancer-cell targeting of the exosomes resulting in enhanced tumor reduction. Milk exosomes exhibited cross-species tolerance with no adverse immune and inflammatory response. Thus, we show the versatility of milk exosomes with respect to the cargo it can carry and ability to achieve tumor targetability. This is the first report to identify a biocompatible and cost-effective means of exosomes to enhance oral bioavailability, improve efficacy and safety of drugs.

KEYWORDS:

Chemopreventive agents; Chemotherapeutic drugs; Drug delivery; Milk-derived exosomes; Tumor-targeting

PMID:
26604130
PMCID:
PMC4706492
DOI:
10.1016/j.canlet.2015.10.020
[Indexed for MEDLINE]
Free PMC Article

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