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Virus Genes. 2016 Feb;52(1):130-3. doi: 10.1007/s11262-015-1262-1. Epub 2015 Nov 24.

Bufavirus Protoparvovirus in feces of wild rats in China.

Author information

1
School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
2
College of Biology and the Environment, Nanjing Forestry University, Nanjing, People's Republic of China.
3
Department of Interventional Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, People's Republic of China.
4
Division of Acute Infectious Disease Prevention and Control, Taizhou Center for Disease Control and Prevention, Taizhou, People's Republic of China.
5
Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA.
6
School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China. z0216wen@yahoo.com.

Abstract

Bufavirus (BuV) was first discovered from feces of children with acute diarrhea. It was subsequently detected from several animal species including shrews, bats, and nonhuman primates. In this study, we identified a novel Protoparvovirus, designated RatBuV, from the intestinal contents of wild rats using viral metagenomics. The near complete genome was 4643 nt encoding NS1, VP1, and VP2 proteins. Phylogenetic analysis over the complete genome showed that RatBuV clustered with Mpulungu BuV from shrews. Sequence analysis indicated that the putative protein sequences of NS1, VP1, and VP2 of RatBuV shared identities of 50.6-77.2, 48.3-77.3, and 47.1-78.3 %, respectively, with those of human BuVs, MpBuV, and WUHARV parvovirus, suggesting RatBuV belongs to a new species of Protoparvovirus. Our epidemiologic study indicated that the prevalence rate of RatBuV in the cohort of 40 wild rats is 12.5 % (5/40), which is higher than that of BuV in humans in a previous study.

KEYWORDS:

Bufavirus; Genome structure; Phylogentic analysis; Wild rats

PMID:
26604002
DOI:
10.1007/s11262-015-1262-1
[Indexed for MEDLINE]

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