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Nat Commun. 2015 Nov 25;6:8881. doi: 10.1038/ncomms9881.

Confocal multiview light-sheet microscopy.

Author information

1
European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
2
European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.

Abstract

Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging with electronic confocal slit detection implemented on modern camera sensors. In addition to improved imaging quality, the electronic confocal slit detection doubles the acquisition speed in multiview setups with two opposing illumination directions allowing simultaneous dual-sided illumination. Confocal multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, easing data handling and storage.

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