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Science. 1989 Jun 16;244(4910):1288-92.

Altering the genome by homologous recombination.

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Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.


Homologous recombination between DNA sequences residing in the chromosome and newly introduced, cloned DNA sequences (gene targeting) allows the transfer of any modification of the cloned gene into the genome of a living cell. This article discusses the current status of gene targeting with particular emphasis on germ line modification of the mouse genome, and describes the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred.

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