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Clinics (Sao Paulo). 2015 Nov;70(11):751-7. doi: 10.6061/clinics/2015(11)07.

Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats.

Author information

1
Department of Pharmacology, Faculty of Medicine, UKMMC, Universiti Kebangsaan Malaysia, Cheras/, Kuala Lumpur, Malaysia.

Abstract

OBJECTIVES:

This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers.

METHODS:

Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration.

RESULTS:

Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO.

CONCLUSION:

The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.

PMID:
26602523
PMCID:
PMC4642492
DOI:
10.6061/clinics/2015(11)07
[Indexed for MEDLINE]
Free PMC Article

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