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Gut Liver. 2016 Jul 15;10(4):624-31. doi: 10.5009/gnl15120.

Ascitic Fluid Calprotectin and Serum Procalcitonin as Accurate Diagnostic Markers for Spontaneous Bacterial Peritonitis.

Author information

1
Department of Tropical Medicine, Mansoura University, Mansoura, Egypt.
2
Department of Clinical Pathology, Mansoura University, Mansoura, Egypt.
3
Department of Endemic Diseases and Gastroenterology, Aswan University, Aswan, Egypt.
4
Department of Internal Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
5
Department of Medical Microbiology and Immunology, Mansoura University, Mansoura, Egypt.

Abstract

BACKGROUND/AIMS:

The diagnosis of spontaneous bacterial peritonitis (SBP) is based on a polymorphonuclear leukocytes (PMNs) exceeding 250/μL in ascitic fluid. The aim of the study was to evaluate serum procalcitonin and ascitic fluid calprotectin as accurate diagnostic markers for detecting SBP.

METHODS:

Seventy-nine patients with cirrhotic ascites were included. They were divided into a SBP group, including 52 patients, and a non-SBP group of 27 patients. Serum procalcitonin, ascitic calprotectin, and serum and ascitic levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL- 6) were measured using an enzyme-linked immunosorbent assay.

RESULTS:

Serum procalcitonin and ascitic calprotectin were significantly higher in SBP patients than in non-SBP patients. Significant increases in both serum and ascitic levels of TNF-α and IL-6 were observed in SBP patients versus non- SBP patients. At a cutoff value of 0.94 ng/mL, serum procalcitonin had 94.3% sensitivity and 91.8% specificity for detecting SBP. In addition, at a cutoff value of 445 ng/mL, ascitic calprotectin had 95.4% sensitivity and 85.2% specificity for detecting SBP. Both were positively correlated with ascitic fluid proteins, PMN count, TNF-α, and IL-6.

CONCLUSIONS:

According to our findings, determination of serum procalcitonin levels and ascitic calprotectin appears to provide satisfactory diagnostic markers for the diagnosis of SBP.

KEYWORDS:

Calprotectin; Interleukin-6; Procalcitonin; Spontaneous bacterial peritonitis; Tumor necrosis factor-alpha

PMID:
26601826
PMCID:
PMC4933425
DOI:
10.5009/gnl15120
[Indexed for MEDLINE]
Free PMC Article

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