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Eur Thyroid J. 2015 Sep;4(Suppl 1):59-66. doi: 10.1159/000437304. Epub 2015 Jul 2.

The Effects of Thyroid Hormones on Gene Expression of Acyl-Coenzyme A Thioesterases in Adipose Tissue and Liver of Mice.

Author information

1
Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
2
Department of Endocrinology and Metabolism, University of Duisburg-Essen, Essen, Germany.
3
Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany ; IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany.
4
Institute for Experimental Endocrinology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
5
Department of Nuclear Medicine, University of Leipzig, Leipzig, Germany.
6
Department of Nuclear Medicine, University of Leipzig, Leipzig, Germany ; IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany.

Abstract

BACKGROUND:

Thyroid hormones (TH) exert pleiotropic effects on glucose and lipid homeostasis. However, it is as yet unclear how TH regulate lipid storage and utilization in order to adapt to metabolic needs. Acyl-CoA thioesterases (ACOTs) have been proposed to play a regulatory role in the metabolism of fatty acids.

OBJECTIVES:

We investigated the interaction between thyroid dysfunction and Acot expression in adipose tissues and livers of thyrotoxic and hypothyroid mice.

METHODS:

Ten-week-old female C57BL/6NTac mice (n = 10/group) were made hyperthyroid by the application of L-thyroxine (2 µg/ml in drinking water) for 4 weeks. Hypothyroidism was induced in 10-week-old mice by feeding an iodine-free chow supplemented with 0.15% PTU for 4 weeks. We measured mRNA expression levels of Acot8, 11 and 13 in the liver and epididymal and inguinal white and brown adipose tissues (BAT). Furthermore, we investigated hepatic Acot gene expression in TRα- and TRβ-deficient mice.

RESULTS:

We showed that the expression of Acot8, 11 and 13 is predominantly stimulated by a thyrotoxic state in the epididymal white adipose tissue. In contrast, hypothyroidism predominantly induces the expression of Acot8 in BAT in comparison with BAT of thyrotoxic and euthyroid mice (p < 0.01). However, no significant changes in Acot expression were observed in inguinal white adipose tissue. In liver, Acot gene expression is collectively elicited by a thyrotoxic state.

CONCLUSIONS:

These data suggest that ACOTs are targets of TH and are likely to influence 3,5,3'-triiodo-L-thyronine-orchestrated mechanisms of lipid uptake, storage and utilization to adapt the regulation of metabolic demands.

KEYWORDS:

Acyl-CoA thioesterases; Brown adipose tissue; Fatty acids; Thyroid hormones

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