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PLoS One. 2015 Nov 23;10(11):e0127662. doi: 10.1371/journal.pone.0127662. eCollection 2015.

Lack of Evidence for Molecular Mimicry in HIV-Infected Subjects.

Author information

1
Dental Clinical Research Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States of America.
2
Department of Medicine, University of California, San Francisco, San Francisco, CA, United States of America.
3
NIH Clinical Center, National Institutes of Health, Bethesda, MD, United States of America.
4
Office of Biological Products, OPQ, CDER, FDA, Silver Spring, MD, United States of America.

Abstract

Previous studies in HIV patients have reported autoantibodies to several human proteins, including erythropoietin (EPO), interferon-α (IFN-α), interleukin-2 (IL-2), and HLA-DR, as potential mediators of anemia or immunosuppression. The etiology of these autoantibodies has been attributed to molecular mimicry between HIV epitopes and self-proteins. Here, the Luciferase Immunoprecipitation System (LIPS) was used to investigate the presence of such autoantibodies in HIV-infected adults. High levels of antibodies to HIV proteins such as capsid (p24), matrix (p17), envelope (gp41), and reverse transcriptase (RT) were detected using LIPS in both untreated and anti-retroviral-treated HIV-infected individuals but not in uninfected controls. LIPS readily detected anti-EPO autoantibodies in serum samples from subjects with presumptive pure red cell aplasia but not in any of the samples from HIV-infected or uninfected individuals. Similarly, subjects with HIV lacked autoantibodies to IFN-α, IL-2, HLA-DR and the immunoglobulin lambda light chain; all purported targets of molecular mimicry. While molecular mimicry between pathogen proteins and self-proteins is a commonly proposed mechanism for autoantibody production, the findings presented here indicate such a process is not common in HIV disease.

PMID:
26599070
PMCID:
PMC4657959
DOI:
10.1371/journal.pone.0127662
[Indexed for MEDLINE]
Free PMC Article

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