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J Cell Biol. 2015 Nov 23;211(4):807-14. doi: 10.1083/jcb.201507108.

Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum.

Author information

1
Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, Columbus, OH 43210.
2
Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210.
3
Department of Zoology, University of Wisconsin-Madison, Madison, WI 53706.
4
Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, Columbus, OH 43210 jontes.1@osu.edu.

Abstract

Cell-cell recognition guides the assembly of the vertebrate brain during development. δ-Protocadherins comprise a family of neural adhesion molecules that are differentially expressed and have been implicated in a range of neurodevelopmental disorders. Here we show that the expression of δ-protocadherins partitions the zebrafish optic tectum into radial columns of neurons. Using in vivo two-photon imaging of bacterial artificial chromosome transgenic zebrafish, we show that pcdh19 is expressed in discrete columns of neurons, and that these columnar modules are derived from proliferative pcdh19(+) neuroepithelial precursors. Elimination of pcdh19 results in both a disruption of columnar organization and defects in visually guided behaviors. These results reveal a fundamental mechanism for organizing the developing nervous system: subdivision of the early neuroepithelium into precursors with distinct molecular identities guides the autonomous development of parallel neuronal units, organizing neural circuit formation and behavior.

PMID:
26598617
PMCID:
PMC4657173
DOI:
10.1083/jcb.201507108
[Indexed for MEDLINE]
Free PMC Article

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