Format

Send to

Choose Destination
Fitoterapia. 2016 Jan;108:73-80. doi: 10.1016/j.fitote.2015.11.003. Epub 2015 Nov 18.

Triterpenoids and an alkamide from Ganoderma tsugae.

Author information

1
School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
2
Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, 80708, Taiwan.
3
Department of Education and Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
4
Faculty of Fragrance and Cosmetics, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 80708, Taiwan. Electronic address: lincna@cc.kmu.edu.tw.

Abstract

Ganoderma tsugae is a medicinal mushroom. In a continual study on the bioactive constituents of this fungus, a new lanostanoid, 3β-acetoxy-16α-hydroxy-24ξ-methyl-5α-lanosta-8,25-dien-21-oic acid, named tsugaric acid F (1) and a novel palmitamide, N-(3'α,4'β-dihydroxy-2'β-(hydroxymethyl)-1'β-(cyclobutyl)palmitamide (2) were isolated and characterized from the fruit bodies of G. tsugae, and three novel seco-lanostanoids, 3,4-seco-8α,9α-epoxy-5α-lanosta-21-oic acid 3,4 lactone (5), 3,4-seco-5β-lanosta-7,9(11),4(29)-trien-3,21-dioic acid-3-methyl ester (6), 3,4-seco-5β-lanosta-7,9(11),4(29)-trien-3,21-dioic acid (7), and a known compound, 3-oxo-5α-lanosta-8-en-21-oic acid (4) were prepared from 3. The structures of new compounds, 1, 2, 5-7 were determined by spectroscopic methods. Compounds 1 and 4 showed inhibitory effects on xanthine oxidase (XO) with an IC50 values of 313.3 ± 80.0 and 43.9 ± 29.9 μM, respectively when 7 exhibited potent inhibitory effect on superoxide anion generation in rat neutrophils stimulated with formyl-Met-Leu-Phe (fMLP)/cytochalasin B (CB) with an IC50 values of 1.3 ± 0.2 μM. Compounds 4-7 showed weak cytotoxic activities against PC3 cells. These results indicated that 4 and 7 may be used as cancer chemopreventive agents.

KEYWORDS:

Anti-inflammatory; Antioxidant; Cytotoxicity; Ganoderma tsugae; Lanostanoid; Seco-lanostanoid

PMID:
26598137
DOI:
10.1016/j.fitote.2015.11.003
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center