Format

Send to

Choose Destination
Food Chem Toxicol. 2015 Dec;86:374-84. doi: 10.1016/j.fct.2015.11.010. Epub 2015 Nov 18.

Analysis of 3-MCPD- and 3-MCPD dipalmitate-induced proteomic changes in rat liver.

Author information

1
Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany. Electronic address: Albert.Braeuning@bfr.bund.de.
2
Federal Institute for Risk Assessment, Department Food Safety, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.

Abstract

3-Monochloropropane-1,2-diol (3-MCPD) and 3-MCPD fatty acid esters are process contaminants in foodstuff which are generated during thermal treatment. Long-term exposure to 3-MCPD or 3-MCPD esters causes toxicity especially in kidney and testis. 3-MCPD esters are efficiently hydrolyzed in the gastrointestinal tract, suggesting that their toxicity is mediated by free 3-MCPD. Combined exposure to free 3-MCPD and 3-MCPD released from 3-MCPD esters might lead to dietary consumption above the tolerable daily intake of 2 μg/kg body weight/day. Suspected mechanisms of 3-MCPD toxicity include the inhibition of glycolysis and oxidative stress. Here, a comparative proteomic approach was followed to analyze the effects of 3-MCPD or 3-MCPD dipalmitate in livers from rats exposed to 10 mg/kg body weight 3-MCPD, an equimolar dose of 3-MCPD dipalmitate, or a 4-fold lower dose of the ester during a 28-day repeated-dose feeding study. Early cellular changes were monitored in the absence of overt toxicity. A comprehensive view of 3-MCPD- or 3-MCPD dipalmitate-triggered proteomic changes in rat liver links to previously proposed mechanisms of toxicity and substantially extends our knowledge on molecular hepatic effects of 3-MCPD. Organ-independent marker proteins altered upon 3-MCPD exposure, for example DJ-1/PARK7, were identified by comparison of the proteomic patterns of kidney, testis and liver.

KEYWORDS:

3-Chloro-1,2-monopropanediol; Food carcinogen; Heat-induced process contaminant; Hepatotoxicity

PMID:
26597043
DOI:
10.1016/j.fct.2015.11.010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center