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Sci Rep. 2015 Nov 24;5:17219. doi: 10.1038/srep17219.

An accessory wall teichoic acid glycosyltransferase protects Staphylococcus aureus from the lytic activity of Podoviridae.

Li X1,2, Gerlach D1,2, Du X1,2, Larsen J3, Stegger M3,4, Kühner P1,2, Peschel A1,2, Xia G1,2,5, Winstel V1,2.

Author information

1
Infection Biology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
2
German Center for Infection Research (DZIF), partner site Tübingen, 72076 Tübingen, Germany.
3
Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.
4
Pathogen Genomics Division, Translational Genomics Research Institute, 3051 W Shamrell Blvd, Flagstaff, 86001 Arizona, USA.
5
Institute of Inflammation &Repair, The University of Manchester, Manchester, United Kingdom.

Abstract

Many Staphylococcus aureus have lost a major genetic barrier against phage infection, termed clustered regularly interspaced palindromic repeats (CRISPR/cas). Hence, S. aureus strains frequently exchange genetic material via phage-mediated horizontal gene transfer events, but, in turn, are vulnerable in particular to lytic phages. Here, a novel strategy of S. aureus is described, which protects S. aureus against the lytic activity of Podoviridae, a unique family of staphylococcal lytic phages with short, non-contractile tails. Unlike most staphylococcal phages, Podoviridae require a precise wall teichoic acid (WTA) glycosylation pattern for infection. Notably, TarM-mediated WTA α-O-GlcNAcylation prevents infection of Podoviridae while TarS-mediated WTA β-O-GlcNAcylation is required for S. aureus susceptibility to podoviruses. Tracking the evolution of TarM revealed an ancient origin in other staphylococci and vertical inheritance during S. aureus evolution. However, certain phylogenetic branches have lost tarM during evolution, which rendered them podovirus-susceptible. Accordingly, lack of tarM correlates with podovirus susceptibility and can be converted into a podovirus-resistant phenotype upon ectopic expression of tarM indicating that a "glyco-switch" of WTA O-GlcNAcylation can prevent the infection by certain staphylococcal phages. Since lytic staphylococcal phages are considered as anti-S. aureus agents, these data may help to establish valuable strategies for treatment of infections.

PMID:
26596631
PMCID:
PMC4667565
DOI:
10.1038/srep17219
[Indexed for MEDLINE]
Free PMC Article

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