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Development. 2016 Jan 1;143(1):35-44. doi: 10.1242/dev.127233. Epub 2015 Nov 19.

Phosphotyrosyl phosphatase activator facilitates localization of Miranda through dephosphorylation in dividing neuroblasts.

Author information

1
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
2
Institute of Molecular and Cell Biology, ASTAR, 138673 Singapore Temasek Life Sciences Laboratory, 117604 Singapore.
3
Temasek Life Sciences Laboratory, 117604 Singapore.
4
Institute of Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, China.
5
Department of Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, VIC 3083, Australia.
6
Temasek Life Sciences Laboratory, 117604 Singapore Department of Biological Sciences, National University of Singapore, 117543 Singapore.
7
Temasek Life Sciences Laboratory, 117604 Singapore Department of Biological Sciences, National University of Singapore, 117543 Singapore caiyu@tll.org.sg xhyang@zju.edu.cn.
8
College of Life Sciences, Zhejiang University, Hangzhou 310058, China Institute of Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, China caiyu@tll.org.sg xhyang@zju.edu.cn.

Abstract

The mechanism for the basal targeting of the Miranda (Mira) complex during the asymmetric division of Drosophila neuroblasts (NBs) is yet to be fully understood. We have identified conserved Phosphotyrosyl phosphatase activator (PTPA) as a novel mediator for the basal localization of the Mira complex in larval brain NBs. In mutant Ptpa NBs, Mira remains cytoplasmic during early mitosis and its basal localization is delayed until anaphase. Detailed analyses indicate that PTPA acts independent of and before aPKC to localize Mira. Mechanistically, our data show that the phosphorylation status of the T591 residue determines the subcellular localization of Mira and that PTPA facilitates the dephosphorylation of T591. Furthermore, PTPA associates with the Protein phosphatase 4 complex to mediate localization of Mira. On the basis of these results, a two-step process for the basal localization of Mira during NB division is revealed: cortical association of Mira mediated by the PTPA-PP4 complex is followed by apical aPKC-mediated basal restriction.

KEYWORDS:

Asymmetric cell division; Dephosphorylation of Mira; Drosophila neuroblast; PTPA

PMID:
26586222
DOI:
10.1242/dev.127233
[Indexed for MEDLINE]
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