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Methods Mol Biol. 2016;1366:315-325. doi: 10.1007/978-1-4939-3127-9_24.

Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.

Author information

1
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA.
2
Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd., Houston, TX, 77004, USA.
3
Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA.
4
DuPont Haskell Global Centers for Health and Environmental Sciences, Newark, DE, USA.
5
Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd., Houston, TX, 77004, USA. mbondessonbolin@uh.edu.

Abstract

In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.

KEYWORDS:

Estrogen; Fluorescence microscopy; Image analysis; Transgenic zebrafish; Xenoestrogens

PMID:
26585145
DOI:
10.1007/978-1-4939-3127-9_24
[Indexed for MEDLINE]

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