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Methods Mol Biol. 2016;1355:293-306. doi: 10.1007/978-1-4939-3049-4_20.

Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.

Author information

1
Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany.
2
Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany. boris.macek@uni-tuebingen.de.
3
Department of Systems and Synthetic Biology, Chalmers University of Technology, Kemivägen 10, 412 96, Göteborg, Sweden. ivan.mijakovic@chalmers.se.

Abstract

Reversible protein phosphorylation is a key regulatory posttranslational modification that plays a significant role in major cellular signaling processes. Phosphorylation events can be systematically identified, quantified, and localized on protein sequence using publicly available bioinformatic tools. Here we present the software tools commonly used by the phosphoproteomics community, discuss their underlying principles of operation, and provide a protocol for large-scale phosphoproteome data analysis using the MaxQuant software suite.

KEYWORDS:

Bioinformatics; MaxQuant; Phosphorylation site identification

PMID:
26584934
DOI:
10.1007/978-1-4939-3049-4_20
[Indexed for MEDLINE]

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