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Methods Mol Biol. 2016;1355:193-209. doi: 10.1007/978-1-4939-3049-4_13.

Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview.

Author information

1
UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal. ib348@cam.ac.uk.
2
Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK. ib348@cam.ac.uk.
3
UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal.

Abstract

Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4/γ-Fe2O3) superparamagnetic core and a coating shell which presents affinity for a specific target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually first functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specific area, and then with an outer layer of a phosphate-affinity material. Fe3O4-coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.

KEYWORDS:

Affinity; Hybrid material s; Magnetic nanocomposite s; Phosphopeptide enrich ment

PMID:
26584927
DOI:
10.1007/978-1-4939-3049-4_13
[Indexed for MEDLINE]

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