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J Immunol. 2016 Jan 1;196(1):217-31. doi: 10.4049/jimmunol.1501064. Epub 2015 Nov 18.

γδ T Cells Shape Preimmune Peripheral B Cell Populations.

Author information

1
Department of Biomedical Research, National Jewish Health, Denver, CO 80206; Joint Laboratory for Stem Cell Engineering and Technology Transfer, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China;
2
Department of Immunology and Microbiology, University of Colorado Health Sciences Center, Aurora, CO 80045;
3
Department of Biomedical Research, National Jewish Health, Denver, CO 80206;
4
Institute of Food Research and Norwich Medical School, University of East Anglia, Norwich, Norfolk NR4 7UG, United Kingdom; and.
5
Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
6
Department of Biomedical Research, National Jewish Health, Denver, CO 80206; Department of Immunology and Microbiology, University of Colorado Health Sciences Center, Aurora, CO 80045;
7
Department of Biomedical Research, National Jewish Health, Denver, CO 80206; Department of Immunology and Microbiology, University of Colorado Health Sciences Center, Aurora, CO 80045; bornw@njhealth.org.

Abstract

We previously reported that selective ablation of certain γδ T cell subsets, rather than removal of all γδ T cells, strongly affects serum Ab levels in nonimmunized mice. This type of manipulation also changed T cells, including residual γδ T cells, revealing some interdependence of γδ T cell populations. For example, in mice lacking Vγ4(+) and Vγ6(+) γδ T cells (B6.TCR-Vγ4(-/-)/6(-/-)), we observed expanded Vγ1(+) cells, which changed in composition and activation and produced more IL-4 upon stimulation in vitro, increased IL-4 production by αβ T cells as well as spontaneous germinal center formation in the spleen, and elevated serum Ig and autoantibodies. We therefore examined B cell populations in this and other γδ-deficient mouse strains. Whereas immature bone marrow B cells remained largely unchanged, peripheral B cells underwent several changes. Specifically, transitional and mature B cells in the spleen of B6.TCR-Vγ4(-/-)/6(-/-) mice and other peripheral B cell populations were diminished, most of all splenic marginal zone (MZ) B cells. However, relative frequencies and absolute numbers of Ab-producing cells, as well as serum levels of Abs, IL-4, and BAFF, were increased. Cell transfers confirmed that these changes are directly dependent on the altered γδ T cells in this strain and on their enhanced potential of producing IL-4. Further evidence suggests the possibility of direct interactions between γδ T cells and B cells in the splenic MZ. Taken together, these data demonstrate the capability of γδ T cells of modulating size and productivity of preimmune peripheral B cell populations.

PMID:
26582947
PMCID:
PMC4684964
DOI:
10.4049/jimmunol.1501064
[Indexed for MEDLINE]
Free PMC Article

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