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Sci Total Environ. 2016 Feb 1;543(Pt A):135-9. doi: 10.1016/j.scitotenv.2015.11.020. Epub 2015 Nov 12.

Micro-computed tomography (μCT) as a novel method in ecotoxicology--determination of morphometric and somatic data in rainbow trout (Oncorhynchus mykiss).

Author information

1
Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany. Electronic address: markus.brinkmann@bio5.rwth-aachen.de.
2
Department of Experimental Molecular Imaging, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
3
Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
4
Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; College of Resources and Environmental Science, Chongqing University, 1 Tiansheng Road Beibei, Chongqing 400715, China; College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China. Electronic address: henner.hollert@bio5.rwth-aachen.de.

Abstract

Fish are important sentinel organisms for the assessment of water quality and play a central role in ecotoxicological research. Of particular importance to the assessment of health and fitness of fish stocks in response to environmental conditions or pollution are morphometric (e.g. Fulton's condition index) and somatic indices (e.g. hepatosomatic, and gonadosomatic index). Standard measurements of somatic indices are invasive and require, by definition, the sacrifice of examined animals, thus prohibiting longitudinal studies and relocation of animals captured in the field. As a potential solution, in the present study, we propose the use of micro-computed tomography (μCT) as imaging modality to non-invasively tomographically image rainbow trout (Oncorhynchus mykiss) exposed to different sediment suspensions. We here demonstrate that μCT can be used as a tool to reliably measure the volumes of different organs, which could then be applied as a substitute of their weights in calculation of somatic indices. To the best of our knowledge, this study is the first to report the results of μCT analyses in the context of ecotoxicological research in rainbow trout. It has the potential to greatly increase the information value of experiments conducted with fish and also to potentially reduce the number of animals required for studying temporal effects through facilitating longitudinal studies within the same individuals.

KEYWORDS:

Biometry; Condition index; Liver somatic index; Micro-computed tomography

PMID:
26580735
DOI:
10.1016/j.scitotenv.2015.11.020
[Indexed for MEDLINE]

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