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Bioresour Technol. 2016 Jan;200:744-52. doi: 10.1016/j.biortech.2015.10.078. Epub 2015 Oct 28.

Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover.

Author information

1
Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
2
Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
3
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
4
Department of Statistics, The Ohio State University, Columbus, OH 43210, USA.
5
Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA; Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
6
Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA; Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA. Electronic address: yu.226@osu.edu.

Abstract

The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

KEYWORDS:

16S rRNA gene; Illumina sequencing; Metagenomics; Microbiome; Succession

PMID:
26575616
DOI:
10.1016/j.biortech.2015.10.078
[Indexed for MEDLINE]

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