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Mol Plant Pathol. 2016 Aug;17(6):959-72. doi: 10.1111/mpp.12340. Epub 2016 Feb 20.

Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae.

Author information

1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
2
Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
3
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China.
4
Tianjin Crop Research Institute, Tianjin Academy of Agriculture Sciences, Tianjin, 300112, China.
5
Department of Plant Breeding & Genetics, Cornell University, Ithaca, NY, 14853, USA.
6
International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila 1301, Philippines.
7
Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.

Abstract

Resistance in rice cultivars to the rice blast fungus Magnaporthe oryzae is complex and is controlled by both major genes and quantitative trait loci (QTLs). We undertook a genome-wide association study (GWAS) using the rice diversity panel 1 (RDP1) that was genotyped using a high-density (700 000 single nucleotide polymorphisms) array and inoculated with five diverse M. oryzae isolates. We identified 97 loci associated with blast resistance (LABRs). Among them, 82 were new regions and 15 co-localized with known blast resistance loci. The top 72 LABRs explained up to 98% of the phenotypic variation. The candidate genes in the LABRs encode nucleotide-binding site leucine-rich repeat (NBS-LRR) resistance proteins, receptor-like protein kinases, transcription factors and defence-related proteins. Among them, LABR_64 was strongly associated with resistance to all five isolates. We analysed the function of candidate genes underlying LABR_64 using RNA interference (RNAi) technology and identified two new resistance alleles at the Pi5 locus. We demonstrate an efficient strategy for rapid allele discovery using the power of GWAS, coupled with RNAi technology, for the dissection of complex blast resistance in rice.

KEYWORDS:

QTL; SNP; genome-wide association study (GWAS); host resistance; rice blast

PMID:
26574735
DOI:
10.1111/mpp.12340
[Indexed for MEDLINE]

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