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Clin Toxicol (Phila). 2016;54(1):53-5. doi: 10.3109/15563650.2015.1112015. Epub 2015 Nov 17.

Ethanol consumption produces a small increase in circulating miR-122 in healthy individuals.

Author information

1
a Ashworth Laboratories , University of Edinburgh , Edinburgh , UK ;
2
b Edinburgh Medical School , University of Edinburgh , Edinburgh , UK ;
3
c Department of Pharmacology , Toxicology & Therapeutics, BHF Centre for Cardiovascular Science, The Queen's Medical Research Institute, University of Edinburgh , Edinburgh , UK.

Abstract

INTRODUCTION:

MicroRNA 122 (miR-122) is a new circulating biomarker for liver injury, which increases earlier than conventional markers in patients with acetaminophen hepatotoxicity. However, as co-ingestion of ethanol is common with drug overdose, a confounding effect of acute ethanol consumption on serum miR-122 must be examined.

METHODS:

Blood was collected from healthy volunteers before and after recreational consumption of ethanol. Routine biochemistry and haematology measurements were performed, and serum miR-122 was measured by qPCR. The primary outcome was the difference in serum miR-122 with ethanol consumption.

RESULTS:

We recruited 18 participants (72% male). Their mean serum ethanol concentration was 113 mg/dl (95% confidence interval [CI] 91-135 mg/dl) after consuming ethanol. Serum miR-122 increased from a mean of 71.3 million (95% CI 29.3-113.2 million) to 139.1 million (95% CI 62.6-215.7 million) copies/ml (2.2-fold increase). There was no significant difference in serum alanine aminotransferase activity before and after ethanol consumption.

CONCLUSION:

miR-122 increased with moderate ethanol consumption, but the fold change was modest. As increases with acetaminophen toxicity are 100- to 10 000-fold, moderate ethanol intoxication is unlikely to confound the use of this biomarker of hepatotoxicity.

KEYWORDS:

Biomarkers; ethanol; microRNA; toxicity

PMID:
26574140
DOI:
10.3109/15563650.2015.1112015
[Indexed for MEDLINE]
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