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Carbohydr Polym. 2016 Jan 20;136:1065-73. doi: 10.1016/j.carbpol.2015.09.108. Epub 2015 Oct 3.

Exopolysaccharide from Trichoderma pseudokoningii induces the apoptosis of MCF-7 cells through an intrinsic mitochondrial pathway.

Author information

1
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
2
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
3
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
4
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Electronic address: lhx900@aliyun.com.
5
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, China; School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. Electronic address: ksc313@126.com.

Abstract

In this study, we reported the anticancer efficacy of exopolysaccharide (EPS) derived from Trichoderma pseudokoningii, on human breast cancer MCF-7 cells. Our results showed that EPS inhibited the proliferation of MCF-7 cells and induced lactic dehydrogenase release by inducing apoptosis and cell arrest at S phase. Further study revealed that EPS-induced apoptosis of MCF-7 cells was associated with alteration of nuclear morphology, disruption of mitochondrial membrane potential and accumulation of intracellular reactive oxygen species. Sequentially, EPS increased the activation of caspase-9 and caspase-3 in a dose-dependent manner; however, caspase-8 remained intact. Western blot analysis revealed that EPS increased the ratio of Bax/Bcl-2 and promoted the release of cytochrome c into the cytoplasm. Taken together, these findings provided evidence that EPS induced the apoptosis of MCF-7 cells through an intrinsic mitochondrial apoptotic pathway and that EPS may therefore be considered as an effective adjuvant agent against human breast cancer.

KEYWORDS:

Apoptosis; Cell cycle arrest; DCFH-DA (PubChem CID: 77718); Dimethyl sulfoxide (PubChem CID: 679); Exopolysaccharide; Hoechst 33258 (PubChem CID: 5491656); Human breast cancer; JC-1 (PubChem CID: 5492929); Propidium iodide (PubChem CID: 104981); Trichoderma pseudokoningii

PMID:
26572448
DOI:
10.1016/j.carbpol.2015.09.108
[Indexed for MEDLINE]

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