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Carbohydr Polym. 2016 Jan 20;136:875-83. doi: 10.1016/j.carbpol.2015.09.062. Epub 2015 Sep 25.

Characterization of a novel purified polysaccharide from the flesh of Cipangopaludina chinensis.

Author information

1
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, Jiangsu, PR China; School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China. Electronic address: shiyingying82012@163.com.
2
School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
3
Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
4
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, Jiangsu, PR China. Electronic address: cuihao@nju.edu.cn.

Abstract

The purpose of this paper was to investigate the characterization of a novel polysaccharide from the flesh of Cipangopaludina chinensis, named CCPSn. The results found CCPSn was a white powder, readily soluble in hot water and slightly soluble in water. CCPSn was a homopolysaccharide composed of D-glucose (D-Glc) with molecular weight of 91.1 kDa. Based on analysis of UV-visible, FT-IR, periodic acid oxidation, Smith degradation, methylation, GM-MS and NMR, the structure of CCPSn was elucidated as follows: the backbone was composed of (1 → 3) linked α-D-Glc. The branches, consisting of a single (1 → 3) linked α-D-Glc units and terminal α-D-Glc-4-O-SO3(-), were attached to the main chain at C-4 positions. The degree of branching was calculated to be about 16.73%. The C-1 of terminal α-D-Glc-4-O-SO3(-) was linked to O-3 of (1 → 3) linked α-D-Glc in the branches. In addition, the results indicated CCPSn was a sulfated polysaccharide with the sulfate radical content of 9.12%.

KEYWORDS:

Characterization; Cipangopaludina chinensis; Polysaccharide; Purification

PMID:
26572424
DOI:
10.1016/j.carbpol.2015.09.062
[Indexed for MEDLINE]

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