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Carbohydr Polym. 2016 Jan 20;136:177-86. doi: 10.1016/j.carbpol.2015.09.022. Epub 2015 Sep 10.

A procoagulant chemically sulfated mannan.

Author information

1
Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: anagracher@gmail.com.
2
Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: alinebiomed.ufpr@gmail.com.
3
Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: trcipriani@ufpr.br.
4
Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: iacomini@ufpr.br.

Abstract

Disorders of hemostasis can produce innumerous problems. Polysaccharides have been studied both as anticoagulant and as procoagulant agents. A mannan with a main chain of α-(1 → 6)-linked-Manp units, branched at O-2 mainly by side-chains of 2-O-linked-α-Manp units was chemically sulfated, structurally characterized by NMR and GC-MS (methylation, desulfation and methylation with trideuterated iodomethane), and tested in vitro and in vivo on blood coagulation models. Chemical analyses indicate a high degree of substitution on the sulfated polysaccharide. This polymer acted as a procoagulant agent, increasing blood coagulation in normal and hemophilic plasma, activated platelet aggregation and also decreased ex vivo aPTT. Polymers such as the sulfated mannan could be a helpful source of hemostatic agents to prevent hemorrhagic states.

KEYWORDS:

Chemical analysis; Chemically sulfated polysaccharide; Methylation; Procoagulant effect

PMID:
26572344
DOI:
10.1016/j.carbpol.2015.09.022
[Indexed for MEDLINE]

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