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Plant Cell. 2015 Nov;27(11):3245-59. doi: 10.1105/tpc.15.00545. Epub 2015 Nov 13.

A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize.

Author information

1
Centre de Recerca en Agrigenòmica, Consortium CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, 08193 Barcelona, Spain Institute of Plant and Microbial Biology, Academia Sinica, 11529 Taipei, Taiwan.
2
Centre de Recerca en Agrigenòmica, Consortium CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, 08193 Barcelona, Spain.
3
Genomics Unit, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
4
Center for Applied Plant Sciences and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210.
5
Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606.
6
Department of Plant Molecular Genetics, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.
7
Institute of Plant and Microbial Biology, Academia Sinica, 11529 Taipei, Taiwan.
8
Centre de Recerca en Agrigenòmica, Consortium CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, 08193 Barcelona, Spain marta.riera@cragenomica.es david.caparros@cragenomica.es.

Abstract

Lignin is an essential polymer in vascular plants that plays key structural roles in vessels and fibers. Lignification is induced by external inputs such as wounding, but the molecular mechanisms that link this stress to lignification remain largely unknown. In this work, we provide evidence that three maize (Zea mays) lignin repressors, MYB11, MYB31, and MYB42, participate in wound-induced lignification by interacting with ZML2, a protein belonging to the TIFY family. We determined that the three R2R3-MYB factors and ZML2 bind in vivo to AC-rich and GAT(A/C) cis-elements, respectively, present in a set of lignin genes. In particular, we show that MYB11 and ZML2 bind simultaneously to the AC-rich and GAT(A/C) cis-elements present in the promoter of the caffeic acid O-methyl transferase (comt) gene. We show that, like the R2R3-MYB factors, ZML2 also acts as a transcriptional repressor. We found that upon wounding and methyl jasmonate treatments, MYB11 and ZML2 proteins are degraded and comt transcription is induced. Based on these results, we propose a molecular regulatory mechanism involving a MYB/ZML complex in which wound-induced lignification can be achieved by the derepression of a set of lignin genes.

PMID:
26566917
PMCID:
PMC4682300
DOI:
10.1105/tpc.15.00545
[Indexed for MEDLINE]
Free PMC Article

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