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C R Biol. 2015 Dec;338(12):784-92. doi: 10.1016/j.crvi.2015.09.005. Epub 2015 Nov 10.

Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato.

Author information

1
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia. Electronic address: saoussen.benkhedher@gmail.com.
2
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia; Département « génie biotechnologique », école polytechnique de Sousse, 4045 Sousse, Tunisia.
3
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
4
UR13AGR09, Integrated Horticultural Production in the Tunisian Centre East, Regional Center of Researches on Horticulture and Organic Agriculture of Chott-Mariem, University of Sousse, 4042 Chott-Mariem, Tunisia.

Abstract

The aim of this study is to evaluate the efficacy of the strain Bacillus subtilis V26, a local isolate from the Tunisian soil, to control potato black scurf caused by Rhizoctonia solani. The in vitro antifungal activity of V26 significantly inhibited R. solani growth compared to the untreated control. Microscopic observations revealed that V26 caused considerable morphological deformations of the fungal hyphae such as vacuolation, protoplast leakage and mycelia crack. The most effective control was achieved when strain V26 was applied 24h prior to inoculation (protective activity) in potato slices. The antagonistic bacterium V26 induced significant suppression of root canker and black scurf tuber colonization compared to untreated controls with a decrease in incidence disease of 63% and 81%, respectively, and promoted plant growth under greenhouse conditions on potato plants. Therefore, B. subtilis V26 has a great potential to be commercialized as a biocontrol agent against R. solani on potato crops.

KEYWORDS:

Antifungal activity; Bacillus subtilis; Biological control; Potato tuber; Rhizoctonia solani

PMID:
26563555
DOI:
10.1016/j.crvi.2015.09.005
[Indexed for MEDLINE]

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