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Tumour Biol. 2016 Apr;37(4):5275-84. doi: 10.1007/s13277-015-4389-8. Epub 2015 Nov 11.

MicroRNA-150 functions as a tumor suppressor in osteosarcoma by targeting IGF2BP1.

Author information

1
Department of Orthopedics, the Second Hospital of Jilin University, 218 Ziqiang Street, Nanguan District, Changchun, 130042, China.
2
Department of Orthopedics, the Second Hospital of Jilin University, 218 Ziqiang Street, Nanguan District, Changchun, 130042, China. zhaojiawu14208@sohu.com.

Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumor with high morbidity in young adults and adolescents. Increasing evidence has demonstrated that aberrant microRNA (miRNA) expression is involved in OS occurrence and development. miR-150 has been recently widely studied in many cancers, but not including OS. This study is aimed to investigate the expression and biological role of miR-150 in OS. Here, we found that miR-150 expression was consistently downregulated in OS tissues and cell lines compared with the matched adjacent normal tissues and human normal osteoblast cells (NHOst), and its expression was significantly correlated with lymph node metastasis and tumor-node-metastasis (TNM) stage. Functional study showed that restoration of miR-150 expression in OS cells could inhibit cell proliferation, migration, and invasion and induced apoptosis in vitro as well as suppressed tumor growth of OS in vivo. Mechanistically, IGF2 mRNA-binding protein 1(IGF2BP1) was confirmed to act as a direct target of miR-150, and the IGF2BP1 mRNA expression was inversely correlated with the level of miR-150 in OS tissues. In addition, downregulation of endogenous IGF2BP1 exhibited similar effects of overexpression of miR-150. Taken together, these findings suggest that miR-150 functions as a tumor suppressor in OS partially by targeting IGF2BP1.

KEYWORDS:

IGF2BP1; Osteosarcoma; Proliferation; miR-150

PMID:
26561465
DOI:
10.1007/s13277-015-4389-8
[Indexed for MEDLINE]

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