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J Clin Microbiol. 2016 Jan;54(1):216-9. doi: 10.1128/JCM.01944-15. Epub 2015 Nov 11.

Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.

Author information

1
Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
2
Enzynomics, Inc., Daejeon, Republic of Korea.
3
Veterinary College, Chungnam National University, Daejeon, Republic of Korea.
4
Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea leekwn@korea.kr.

Abstract

During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.

PMID:
26560537
PMCID:
PMC4702723
DOI:
10.1128/JCM.01944-15
[Indexed for MEDLINE]
Free PMC Article

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