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J Virol. 2015 Nov 11;90(2):1148-51. doi: 10.1128/JVI.02597-15. Print 2016 Jan 15.

Minor Contribution of Chimeric Host-HIV Readthrough Transcripts to the Level of HIV Cell-Associated gag RNA.

Author information

1
Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands a.o.pasternak@amc.uva.nl.
2
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
3
Department of Experimental Immunology, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands.
4
Department of Global Health, Division of Infectious Diseases, and Amsterdam Institute for Global Health and Development, University of Amsterdam, Amsterdam, The Netherlands Stichting HIV Monitoring, Amsterdam, The Netherlands.
5
Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands.

Abstract

Cell-associated HIV unspliced RNA is an important marker of the viral reservoir. HIV gag RNA-specific assays are frequently used to monitor reservoir activation. Because HIV preferentially integrates into actively transcribed genes, some of the transcripts detected by these assays may not represent genuine HIV RNA but rather chimeric host-HIV readthrough transcripts. Here, we demonstrate that in HIV-infected patients on suppressive combination antiretroviral therapy, such host-derived transcripts do not significantly contribute to the HIV gag RNA level.

PMID:
26559833
PMCID:
PMC4702708
DOI:
10.1128/JVI.02597-15
[Indexed for MEDLINE]
Free PMC Article

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