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Biomaterials. 2016 Jan;76:282-91. doi: 10.1016/j.biomaterials.2015.10.068. Epub 2015 Oct 27.

Cell mechanosensory recognizes ligand compliance at biomaterial interface.

Author information

1
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53 80125 Napoli, Italy; Interdisciplinary Research Centre on Biomaterials (CRIB) University of Naples Federico II Piazzale Tecchio 80, 80125 Napoli, Italy.
2
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53 80125 Napoli, Italy; Interdisciplinary Research Centre on Biomaterials (CRIB) University of Naples Federico II Piazzale Tecchio 80, 80125 Napoli, Italy; Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale (DICMAPI), University "Federico II", Piazzale Tecchio 80, 80125 Naples, Italy. Electronic address: causa@unina.it.
3
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53 80125 Napoli, Italy; Interdisciplinary Research Centre on Biomaterials (CRIB) University of Naples Federico II Piazzale Tecchio 80, 80125 Napoli, Italy; Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale (DICMAPI), University "Federico II", Piazzale Tecchio 80, 80125 Naples, Italy.

Abstract

Cells activate signalling through ligand-receptor bonds by sensing the mechanical properties of the surrounding extracellular matrix (ECM). Ligands, indeed, have to withstand the pulling force elicited by cell receptors through focal adhesions (FAs). On this basis, we developed functional ligands to be simply adsorbed on surfaces and constituted by a two-domain peptide: one derived from ECM proteins and available to receptors to offer biochemical cues, and another adsorbed on material to withstand the tension upon receptor engagement. Tuneable compliance of the anchoring domain of the peptide ligand was verified by single peptide analysis through molecular dynamics and adsorption measurements. We showed that the highest adsorbed peptides combined with integrin cell-binding motifs allow for the cell recognition and polarization with larger mature FA areas. On the contrary, the lowest adsorbed sequences did not provide mechanical resistance to the integrin pulling action, leading to more rounded cells with smaller FA areas. This evidence demonstrates that cell mechanosensory can discriminate ligands on surfaces and should be considered as a criterion in ligand design for material bioactivation.

KEYWORDS:

Ligand; Material bioactivation; Mechanosensory; Peptide

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