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Methods. 2016 Feb 15;95:55-61. doi: 10.1016/j.ymeth.2015.11.006. Epub 2015 Nov 10.

Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.

Author information

1
School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, United Kingdom. Electronic address: trushar7@hotmail.com.
2
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
3
National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, United Kingdom; ISIS Spallation Neutron and Muon Source and Research Complex at Harwell, Rutherford Appleton Laboratory, Oxfordshire OX11 0FA, United Kingdom. Electronic address: David.Scott@nottingham.ac.uk.

Abstract

Analytical ultracentrifugation, an early technique developed for characterizing quantitatively the solution properties of macromolecules, remains a powerful aid to structural biologists in their quest to understand the formation of biologically important protein complexes at the molecular level. Treatment of the basic tenets of the sedimentation velocity and sedimentation equilibrium variants of analytical ultracentrifugation is followed by considerations of the roles that it, in conjunction with other physicochemical procedures, has played in resolving problems encountered in the delineation of complex formation for three biological systems - the cytoplasmic dynein complex, mitogen-activated protein kinase (ERK2) self-interaction, and the terminal catalytic complex in selenocysteine synthesis.

KEYWORDS:

Analytical ultracentrifugation; Catalytic complex in selenocysteine synthesis; Cytoplasmic dynein complex; Mitogen-activated protein kinase (ERK2); Sedimentation equilibrium; Sedimentation velocity; SepSecS–tRNA(Sec) interaction

PMID:
26555086
DOI:
10.1016/j.ymeth.2015.11.006
[Indexed for MEDLINE]

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