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Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14688-93. doi: 10.1073/pnas.1508114112. Epub 2015 Nov 9.

Asymptomatic humans transmit dengue virus to mosquitoes.

Author information

1
Virology Unit, Institut Pasteur in Cambodia, International Network of Pasteur Institutes, 12201 Phnom Penh, Cambodia;
2
Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France; Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris, France;
3
Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris, France; Functional Genetics of Infectious Diseases Unit, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris, France;
4
Epidemiology and Public Health Unit, Institut Pasteur in Cambodia, International Network of Pasteur Institutes, 12201 Phnom Penh, Cambodia;
5
Department of Entomology and Nematology, University of California, Davis, CA 95616;
6
National Center for Malariology, Ministry of Health, 12302 Phnom Penh, Cambodia;
7
Department of Entomology and Nematology, University of California, Davis, CA 95616; Fogarty International Center, National Institutes of Health, Bethesda, MD 20892;
8
Virology Unit, Institut Pasteur in Cambodia, International Network of Pasteur Institutes, 12201 Phnom Penh, Cambodia; GlaxoSmithKline, Vaccines Value Health Sciences, Singapore 189720 buchyphilippe@hotmail.com.

Abstract

Three-quarters of the estimated 390 million dengue virus (DENV) infections each year are clinically inapparent. People with inapparent dengue virus infections are generally considered dead-end hosts for transmission because they do not reach sufficiently high viremia levels to infect mosquitoes. Here, we show that, despite their lower average level of viremia, asymptomatic people can be infectious to mosquitoes. Moreover, at a given level of viremia, DENV-infected people with no detectable symptoms or before the onset of symptoms are significantly more infectious to mosquitoes than people with symptomatic infections. Because DENV viremic people without clinical symptoms may be exposed to more mosquitoes through their undisrupted daily routines than sick people and represent the bulk of DENV infections, our data indicate that they have the potential to contribute significantly more to virus transmission to mosquitoes than previously recognized.

KEYWORDS:

Aedes aegypti; Cambodia; dengue; human-to-mosquito transmission; mosquito experimental infection

PMID:
26553981
PMCID:
PMC4664300
DOI:
10.1073/pnas.1508114112
[Indexed for MEDLINE]
Free PMC Article

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