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Methods Mol Biol. 2016;1380:61-75. doi: 10.1007/978-1-4939-3197-2_5.

Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C.

Author information

1
Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
2
Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.

Abstract

Capillary electrophoresis-based SELEX (CE-SELEX) is an efficient technique for the isolation of aptamers binding to a wide range of target molecules. CE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be performed on non-immobilized targets, usually within a fewer number of selection cycles. Here we describe a complete procedure of CE-SELEX using activated protein C (APC) as the target protein.

KEYWORDS:

Activated protein C; Aptamer; CE-SELEX; Capillary electrophoresis; SELEX

PMID:
26552816
DOI:
10.1007/978-1-4939-3197-2_5
[Indexed for MEDLINE]

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