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Zhonghua Nan Ke Xue. 2015 Sep;21(9):788-91.

[Indirubin inhibits the proliferation of prostate cancer PC-3 cells].

[Article in Chinese]

Abstract

OBJECTIVE:

To explore the antitumoral effect of indirubin on androgen-independent prostate cancer PC-3 cells and its possible mechanisms.

METHODS:

We measured the inhibitory effect of indirubin on the proliferation of prostate cancer PC-3 cells using MTT assay, detected their cell cycles by flow cytometry, and determined the expressions of the cell cycle regulatory protein cyclin D1 and its related downstream gene c-myc by Western blot.

RESULTS:

The viability of the PC-3 cells was significantly decreased by indirubin in a concentration-dependent manner, reduced to 52. 2% and 13. 6% at 5 and 10 ┬Ámol/L, respectively. The cell cycle of the PC-3 cells was markedly inhibited by indirubin at 5 ┬Ámol/L, with the cells remarkably increased in the G0 and G1 phases and decreased in the S and G2/M phases. Meanwhile, indirubin also inhibited the expressions of cyclin D1 and c-myc in the Wnt signaling pathway.

CONCLUSION:

Indirubin can suppress the proliferation of androgen-independent prostate cancer PC-3 cells, which may be associated with its inhibitory effect on the cell cycle and Wnt signaling pathway.

PMID:
26552210
[Indexed for MEDLINE]

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