Send to

Choose Destination
Zhonghua Nan Ke Xue. 2015 Sep;21(9):788-91.

[Indirubin inhibits the proliferation of prostate cancer PC-3 cells].

[Article in Chinese]



To explore the antitumoral effect of indirubin on androgen-independent prostate cancer PC-3 cells and its possible mechanisms.


We measured the inhibitory effect of indirubin on the proliferation of prostate cancer PC-3 cells using MTT assay, detected their cell cycles by flow cytometry, and determined the expressions of the cell cycle regulatory protein cyclin D1 and its related downstream gene c-myc by Western blot.


The viability of the PC-3 cells was significantly decreased by indirubin in a concentration-dependent manner, reduced to 52. 2% and 13. 6% at 5 and 10 ┬Ámol/L, respectively. The cell cycle of the PC-3 cells was markedly inhibited by indirubin at 5 ┬Ámol/L, with the cells remarkably increased in the G0 and G1 phases and decreased in the S and G2/M phases. Meanwhile, indirubin also inhibited the expressions of cyclin D1 and c-myc in the Wnt signaling pathway.


Indirubin can suppress the proliferation of androgen-independent prostate cancer PC-3 cells, which may be associated with its inhibitory effect on the cell cycle and Wnt signaling pathway.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center