Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Immunol. 2016 Jan;17(1):104-12. doi: 10.1038/ni.3314. Epub 2015 Nov 9.

The cytotoxic T cell proteome and its shaping by the kinase mTOR.

Author information

1
Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dow Street, Dundee, UK.
2
Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, UK.
3
Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, UK.

Abstract

We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes, and mTORC1 selectively repressed and promoted expression of a subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for negative control of the production of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) by mTORC1 in CTLs. mTORC1 repressed PtdIns(3,4,5)P3 production and determined the requirement for mTORC2 in activation of the kinase Akt. Our unbiased proteomic analysis thus provides comprehensive understanding of CTL identity and the control of CTL function by mTORC1.

Comment in

PMID:
26551880
PMCID:
PMC4685757
DOI:
10.1038/ni.3314
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center