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Environ Microbiol Rep. 2016 Feb;8(1):85-90. doi: 10.1111/1758-2229.12354. Epub 2015 Dec 21.

The Pseudomonas aeruginosa AmrZ C-terminal domain mediates tetramerization and is required for its activator and repressor functions.

Author information

1
Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA.
2
Center for Microbial Interface Biology, The Ohio State University, Columbus, OH, 43210, USA.
3
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA.
4
Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH, 43210, USA.
5
Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
6
Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Wexner Medical Center and Comprehensive Cancer Center, The Ohio State University, Columbus, OH, 43210, USA.
7
Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, 43210, USA.

Abstract

Pseudomonas aeruginosa is an important bacterial opportunistic pathogen, presenting a significant threat towards individuals with underlying diseases such as cystic fibrosis. The transcription factor AmrZ regulates expression of multiple P. aeruginosa virulence factors. AmrZ belongs to the ribbon-helix-helix protein superfamily, in which many members function as dimers, yet others form higher order oligomers. In this study, four independent approaches were undertaken and demonstrated that the primary AmrZ form in solution is tetrameric. Deletion of the AmrZ C-terminal domain leads to loss of tetramerization and reduced DNA binding to both activated and repressed target promoters. Additionally, the C-terminal domain is essential for efficient AmrZ-mediated activation and repression of its targets.

PMID:
26549743
PMCID:
PMC4769699
DOI:
10.1111/1758-2229.12354
[Indexed for MEDLINE]
Free PMC Article

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