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Chem Biol. 2015 Nov 19;22(11):1521-30. doi: 10.1016/j.chembiol.2015.09.015. Epub 2015 Nov 5.

Host-Microbe Protein Interactions during Bacterial Infection.

Author information

1
Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA.
2
Departments of Medicine and Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA.
3
Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA; Departments of Medicine and Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA; Department of Genome Sciences, University of Washington School of Medicine, 850 Republican Street, Brotman Building, Room 154, Seattle, WA 98109, USA. Electronic address: jimbruce@u.washington.edu.

Abstract

Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical crosslinking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with Acinetobacter baumannii. These efforts resulted in identification of 3,076 crosslinked peptide pairs and 46 interspecies protein-protein interactions. Most notably, the key A. baumannii virulence factor, OmpA, was identified as crosslinked to host proteins involved in desmosomes, specialized structures that mediate host cell-to-cell adhesion. Co-immunoprecipitation and transposon mutant experiments were used to verify these interactions and demonstrate relevance for host cell invasion and acute murine lung infection. These results shed new light on A. baumannii-host protein interactions and their structural features, and the presented approach is generally applicable to other systems.

PMID:
26548613
PMCID:
PMC4756654
DOI:
10.1016/j.chembiol.2015.09.015
[Indexed for MEDLINE]
Free PMC Article
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