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Microscopy (Oxf). 2016 Feb;65(1):35-41. doi: 10.1093/jmicro/dfv355. Epub 2015 Nov 6.

Single-particle cryo-EM data acquisition by using direct electron detection camera.

Author information

1
Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
2
Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA Howard Hughes Medical Institute, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA ycheng@ucsf.edu.

Abstract

Recent advances in single-particle electron cryo-microscopy (cryo-EM) were largely facilitated by the application of direct electron detection cameras. These cameras feature not only a significant improvement in detective quantum efficiency but also a high frame rate that enables images to be acquired as 'movies' made of stacks of many frames. In this review, we discuss how the applications of direct electron detection cameras in cryo-EM have changed the way the data are acquired.

KEYWORDS:

direct electron detection camera; electron cryo-microscopy; single-particle cryo-EM

PMID:
26546989
PMCID:
PMC4749049
DOI:
10.1093/jmicro/dfv355
[Indexed for MEDLINE]
Free PMC Article

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