The impact of calicivirus mixed infection in an oyster-associated outbreak during a food festival

J Clin Virol. 2015 Dec:73:55-63. doi: 10.1016/j.jcv.2015.10.004. Epub 2015 Oct 8.

Abstract

Background: Despite calicivirus food-borne outbreaks posing major public health concern worldwide, little information is at present available about the impact of caliciviruses mixed infection in an oyster-associated outbreak in China.

Objectives: To investigate the clinical and epidemiologic characteristics of an oyster-associated calicivirus outbreak initiated by a food festival in Shanghai, China, in April 2014.

Study design: Molecular epidemiological studies based on nucleotide sequencing and phylogenetic analysis of calicivirus strains from patients.

Results: A total of 65 of the 78 (83%) cases from this outbreak were associated with raw oyster consumption. Forty-six calicivirus strains were identified from 25 stool specimens with norovirus (NoV) GII.4 Sydney_2012, GII.13, GI.2, GI.5 and sapovirus (SaV) GI.2 being predominant genotypes and with a prevalence of triple-, double- and single-infection being 20%, 48% and 28%, respectively. Meanwhile, 13 putative NoV recombinants were indicated by the phylogenetic inconsistency between capsid and polymerase genotype, mainly including GII.Pe/GII.4 Sydney_2012. Molecular epidemiological investigation showed possible multiple route transmission in the field. The clinical and epidemiologic characteristics of the mixed point-source calicivirus outbreak also conformed to Kaplan's criteria.

Conclusions: This is the first reported oyster-associated calicivirus outbreak with a high prevalence of mixed infection during a food festival described in China. Our investigation underscores the importance of early surveillance and comprehensive etiologic identification of mixed point-source outbreaks and the need for reliable standards of monitoring oysters to prevent and control calicivirus food-borne outbreaks in China.

Keywords: Mixed infection; Molecular epidemiology; Norovirus; Oyster-associated outbreak; Phylogenetic analysis; Sapovirus.

MeSH terms

  • Adult
  • Animals
  • Caliciviridae / classification*
  • Caliciviridae / genetics
  • Caliciviridae / isolation & purification*
  • Caliciviridae Infections / epidemiology*
  • Caliciviridae Infections / virology
  • China / epidemiology
  • Coinfection / epidemiology
  • Disease Outbreaks
  • Female
  • Food Microbiology
  • Humans
  • Male
  • Middle Aged
  • Ostreidae / virology*
  • Phylogeny
  • RNA, Viral / analysis
  • Sequence Analysis, RNA
  • Young Adult

Substances

  • RNA, Viral