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Respirology. 2016 Feb;21(2):321-8. doi: 10.1111/resp.12677. Epub 2015 Nov 6.

Clinical utility of rapid pathogen identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in ventilated patients with pneumonia: A pilot study.

Author information

1
Department of Internal Medicine, Pusan National University School of Medicine, Busan, Korea.
2
Biomedical Research Institute, Pusan National University Hospital, Busan, Korea.
3
Department of Laboratory Medicine, Pusan National University School of Medicine, Busan, Korea.

Abstract

BACKGROUND AND OBJECTIVE:

We evaluated the clinical utility of rapid identification of microorganisms in bronchoalveolar lavage (BAL) fluid using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in terms of the clinical outcomes of ventilated patients with pneumonia.

METHODS:

Patients for whom microorganisms were identified via MALDI-TOF MS (from March 2013 to February 2014; post-intervention group) were compared with patients for whom microorganisms were identified using conventional methods (from March 2012 to February 2013; pre-intervention group). All pneumonia types (community-acquired, hospital-acquired, healthcare-associated and ventilator-associated pneumonia) were included in the analysis.

RESULTS:

In total, 77 patients (50 men, mean age 67.2 ± 12.5 years) were included (40 patients in the pre-intervention group and 37 in the post-intervention group). The time from BAL fluid collection to microorganism identification and the availability of antimicrobial susceptibility results was shorter in the post- compared with the pre-intervention group (51.9 ± 11.3 vs 67.3 ± 17.4 h, P < 0.001). Also, the time from BAL fluid collection to adjustment of antibiotic therapy was shorter in the post-intervention group (56.5 ± 10.9 vs 73.2 ± 18.5 h, P < 0.001). Microorganism identification via MALDI-TOF MS was independently associated with a shorter intensive care unit (ICU) stay after BAL fluid was drawn (hazard ratio = 2.324, P = 0.007).

CONCLUSION:

Rapid identification of microorganisms in BAL fluid via MALDI-TOF MS was associated with adjustment of antibiotic therapy and a shorter ICU stay after BAL fluid was collected from ventilated patients with pneumonia.

KEYWORDS:

bronchoalveolar lavage; identification; matrix-assisted laser desorption mass spectrometry; pneumonia; ventilator

PMID:
26545565
DOI:
10.1111/resp.12677
[Indexed for MEDLINE]
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